Can anyone help with an orbitrap mass spectrometer. Thermo scientific q exactive focus hybrid quadrupole. Matthew robey software engineer proteinaceous, inc. Thermo scientific q exactive orbitrap lcmsms system. Thermo scientific q exactive hf orbitrap lcmsms system. The thermo scientific tm q exactive tm hybrid quadrupoleorbitrap mass spectrometer introduced the revolutionary concept of quanfirmation tm. The thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer introduced the revolutionary concept of quanfirmation. The thermo scientific tm q exactive tm plus takes quanfirmation to a. Identify, quantify and confirm more compounds rapidly and with confidence using the thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer. Converting thermo q exactive files for use in proteinpilot. Q exactive hybrid quadrupoleorbitrap mass spectrometer.
How much orbitrap q exactive plus costs compared to orbitrap exactive plus. When combined with thermo scientific biopharma finder software it. Thermo fisher scientific quadrupole q1 access mass filter assembly. I am currently working with a q exactive focus from thermo and within 3. The thermo scientific q exactive hybrid quadrupoleorbitrapmass. How much orbitrap q exactive plus costs compared to. Resolving power up to 140,000 maximum scan speed 12 hz intrascan dynamic range 5000.
Q exactive hf is a member of the thermo scientific family of mass spectrometer ms detectors that are powered by orbitrap technology. The q exactive focus system simplifies method development, saving time and decreasing costs while reliably delivering unsurpassed results. The new thermo scientific q exactive hfx hybrid quadrupoleorbitrap mass spectrometer is two times faster than its predecessor, with improved ion transmission. Check the connected ion source on mass spec if it is the correct ion source for your experiment. Tmt workflow on the q exactive series thermo fisher scientific. Exactive preinstallation requirements guide exactive software manual exactive quickstart guide ion max and ion maxs api source hardware manual. Product specifications thermo scientific q exactive. Exactive is a member of the thermo scientific family of mass spectrometer ms detectors that are powered by orbitrap technology. Food and drug administration and state and local regulatory agencies. You can use the instrument control software to collect highquality mass spectrometry data on the thermo scientific mass spectrometers. The attached minitutorials explain how to set up dia methods for the thermo q exactive instrument.
Find methods for your needs thermo fisher scientific. Q exactive hf software manual thermo fisher scientific. For questions about the software, to request features, or to report defects, please email planet. Andrea brunner of kwr discusses emerging contaminants and associated challenges and solutions of nontargeted screening for environmental water quality monitoring. Thermo fisher q exactive focus mass spectrometer slides.
A comprehensive understanding of samples has been out of reach for gcms users for too long. Thermo scientific dionex chromeleon chromatography data system cds software. Q exactive hybrid quadrupole orbitrap mass spectrometer. The food and drug administration fda released a liquid chromatographyhigh resolution mass spectrometry lchrms method for the quantitation of nnitrosodimethylamine ndma in ranitidine drug substance and drug products using the q exactive hybrid quadrupoleorbitrap mass spectrometer. Q exactive hybrid quadrupoleorbitrap mass spectrometer support. The q exactive uhmr hybrid quadrupoleorbitrap mass spectrometer is thermo fisher scientifics first mass spectrometry platform to combine substantially increased sensitivity and mass resolution at high mz, with ms 2 and pseudoms 3 capabilities creating a unique and powerful analytical solution for the investigation of structure and heterogeneity of protein complexes. Thermo fisher scientific exactive software manual pn 1249910, revision c i read this first welcome to the thermo scientific exactive system. Control of the instruments is through two application packages. The company will showcase the applications software, as well as the q exactive mass spectrometer, in thermo scientific booth 1 and at the thermo scientific hospitality suite at the vancouver convention center parkview terrace rooms.
Thermo scientific q exactive hf orbitrap lcmsms system higherquality data, faster than ever the thermo scientific q exactive hf benchtop lcmsms combines a highresolution accuratemass hram ultrahighfield orbitrap analyzer and a high performance quadrupole precursor ion selection to deliver unsurpassed speed while. Thermo fisher scientific exactive operating manual iii related documentation in addition to this guide, thermo fisher scientific provides the following documents for exactive. Q exactive orbitrap mass spectrometers thermo fisher scientific. This q exactive hf software manual is intended for all personnel that need to perform measurements with the q exactive hf mass spectrometer, especially the. Thermo scientific q exactive hf software manual pn 72090, revision a i read this first welcome to the thermo scientific q exactive hf system. Identify, quantify and confirm more compounds rapidly and with confidence using the thermo scientific q exactive hy. Thermo scientific q exactive plus orbitrap lcmsms system characterize, quantify and confirm with unmatched confidence the thermo scientific q exactive plus benchtop lcmsms combines highperformance quadrupole precursor selection with highresolution, accuratemass hram orbitrap detection to deliver. Products orbitrap eclipse orbitrap exploris 480 orbitrap fusion lumos orbitrap fusion orbitrap idx q exactive uhmr q exactive. Close integration with applicationspecific software ensures maximum productivity. The superior quality of q exactive plus msms data enables identification and quantitation of more compounds with greater confidence. Our core is deciding between the purchase or lease of a. Can anyone help with an orbitrap mass spectrometer calibration issue.
Compound discoverer software helps you extract highconfidence. Q exactive plus hybrid quadrupoleorbitrap mass spectrometer. The thermo scientific q exactive plus benchtop orbitrap mass spectrometer driver has been updated to improve. Does anyone have an opinion on whether the thermo q. Combined with thermo scientific tracefinder software, the q exactive focus ms offers a powerful, yet simple to use, solution for routine analysis laboratories. Protein mode on the q exactive hf is a powerful combination that has set the standard for the highest quality isotopically resolved data achievable on a benchtop mass spectrometer. Our core is deciding between the purchase or lease of a thermo q exactive or waters synapt g2s. Thermo scientific q exactive benchtop quadrupoleorbitrap mass spectrometer identify, quantify and confirm with unmatched confidence using the q exactive mass spectrometer. Nontargeted screening for environmental water quality. Aligned maximum injection and agc target settings for multiplexed scans with q exactive and orbitrap exploris 480 ms systems.
Thermo exactive the exactive plus orbitrap lcms is a benchtop full scan mass spectrometer designed for compound identification and highthroughput, highperformance screening. What are the advantagesdisadvantages of using tsimdd. Exactive operating manual thermo fisher scientific. These courses blend classroom training with handson lab exercises, and cover various topics including theory, software familiarization, instrument tuning and calibration, and workflows, offered at sciex locations across the globe. In addition, there is a range of softwares, such as thermo scientific proteome discoverer, sieve software, and pinpoint software for supporting the proteomics research. Thermo scientific exactive series operating manual pn bre0012255, revision a vii. Thermo scientific q exactive plus orbitrap lcmsms system. Thermo scientific quadrupole q1 access orbitrap mass. Q exactive product specialist, thermo fisher scientific, germany. Biopharma characterization with the thermo scientific q exactive biopharma.
Exactive gc orbitrap gcms and q exactive gc orbitrap gc. The company will showcase the applications software, as well as the q exactive mass spectrometer, in thermo scientific booth 1 and at the thermo scientific hospitality suite at the vancouver convention center parkview terrace rooms 1, 2 and 3 during asms 2012, being held from may 21 24. Please contact us for complete refurbishedused thermo q exactive mass spectrometer system, warranty and service contract details. What are the advantagesdisadvantages of using tsimddms2 versus prm on the q exactive. Here we present a method for screening using a diluteandshoot approach with the q exactive focus mass spectrometer and thermo scientific toxfinder software. The dia data collected on the q exactive ms can be processed with thermo scientific pinpoint software 1. In addition, metworks software allows highresolution, accuratemass data from orbitrap, ltq ft, q exactive and exactive instruments to be fully leveraged. Utilization of high resolution lcms for screening and quantitative analysis of pesticides in food matrix using a q exactive bench top orbitrap platform.
The q exactive plus hybrid quadrupoleorbitrap mass spectrometer takes quanfirmation to the next level with. The q exactive lcmsms combines the srm and hram orbitrap technology, making it possible for the clinical research and forensic. Q exactive focus orbitrap lcmsms system thermo fisher. The thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer combines highperformance quadrupole precursor selection with highresolution, accuratemass hram orbitrap detection. The q exactive plus lcmsms system not only offers broad screening capabilities but also excels at targeted quantitation experiments. Thermo fisher scientific quad q exactive access orbitrap mass. Q exactive biopharma platform thermo fisher scientific. With a fast scan speed and multiplexing capabilities, the q exactive mass spectrometer is an outstanding detector for. The log tells us that the ms disconnected since a few days ago. Net assembly for reading our raw, sld, and pmd files. Deconvolution of subunit ms spectra is easy and powerful with thermo scientific biopharma finder software.
Refurbished thermo scientific q exactive hybrid quadrupoleorbitrap mass spectrometer. Instrument parameter optimization and data analysis in proteome discoverer 2. Development of a new hybrid quadrupoleorbitrap mass spectrometer with improved performance results. Q exactive hf tune to set up the mass spectrometer in the q exactive hf tune software for tuning and calibration in the hesims mode 1. Thermo fisher q exactive focus mass spectrometer slides full screen talkthetalktv. The q exactive benchtop lcmsms combines highperformance quadrupole precursor selection with highresolution, accuratemass hram orbitrap detection to deliver high performance and tremendous versatility.
However, i wish there was more support from the manufacturer after purchase for various issues weve run into with bad parts. Identify, quantify and confirm more compounds rapidly and with confidence using the thermo scientific q exactive hybrid quadrupoleorbitrap mass. Thermo fisher scientific announces expanded portfolio of. Thermo fisher orbitrap instrument control software. The superior quality of q exactive msms data provides ultimate confidence for a wide range of qualitative and quantitative applications. If you have not already done so, choose start programs thermo exactive series tune to start the q exactive hf tune program. We are not sure if this is a hardware or software issue. Thermo fisher scientific introduces instrument control. Applicationspecific software is available to make full use of the power of the q exactive focus instrument. The sale of this item may be subject to regulation by the u. The thermo scientific q exactive gc orbitrap gcmsms system and the new thermo scientific exactive gc orbitrap gcms system have changed all of that. It is simple to use and maintenance on the q exactive is usually straightforward when outlined in the manual. Thermaqs large backlit display delivers the right temperature info without having to push confusing buttons and with none of the useless fluff found in houseware products.
Created software for similarity scoring and clustering of metabolomics mass spectra. The third yellow lights is on suggesting performance issue calibration is needed usually 7. Q exactive orbitrap mass spectrometers thermo fisher. Forensic toxicologists need an economical solution to screen for a virtually unlimited number of compounds in urine. Thermo fisher orbitrap related software planet orbitrap. Metworks software is compatible with all thermo scienti. Powerful integrated software for characterization of biotherapeutics. Below are some good starting settings to convert q exactive files to mgf using proteowizard, for searching with proteinpilot software. The dia acquisition strategy adds another multiplexing method to the q exactive instrument, which already allows users to multiplex up to 10 precursor ions for quantification in full ms and msms mode. This benchtop lcmsms system combines quadruple precursor ion selection with highresolution, accuratemass hram orbitrap detection to del.